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This collection showcases articles published in PLOS ONE that apply mathematics to biological questions. As the selection shows, this field is very broad, spanning topics such as angiogenesis, morphogenesis, cell dynamics and interactions, metabolism modelling, biochemical processes, and touches on all scales of biological organization. In this Editor’s Picks, PLOS ONE Associate Editor Carla Pegoraro highlights some of the latest research on mathematical and physical biology. We welcome submissions in this field and hope to grow our collection over time.