Biology & Life Sciences

Editor’s Picks: Molecular Biology & Biochemistry

Published October 6, 2017 Curated Collections

Molecular Biology and Biochemistry form the backbone of biomedical research in the post-genomic era. This collection, as selected by PLOS ONE Associate Editor Adya Misra, highlights publications that represent recent developments in molecular biology with a special focus on genome editing and high throughput sequencing. Also selected are articles that focus on cell signalling during exercise and metabolic dysfunction, highlighting the importance of utilizing biochemical and molecular biology tools in tandem.

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Genome Editing
High Throughput Sequencing
Cell Signalling in Exercise & Metabolic Dysfunction

Genome Editing

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10.1371/journal.pone.0184009

PLOS ONE Cas9/gRNA targeted excision of cystic fibrosis-causing deep-intronic splicing mutations restores normal splicing of CFTR mRNA
September 1, 2017 David J. Sanz, Jennifer A. Hollywood, Martina F. Scallan, Patrick T. Harrison
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10.1371/journal.pgen.1006796

PLOS Genetics Novel CRISPR/Cas9 gene drive constructs reveal insights into mechanisms of resistance allele formation and drive efficiency in genetically diverse populations
July 20, 2017 Jackson Champer, Riona Reeves, Suh Yeon Oh, Chen Liu, Jingxian Liu, Andrew G. Clark, Philipp W. Messer
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10.1371/journal.pone.0144970

PLOS ONE Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions
January 20, 2016 Yi Li, Saurabh Mendiratta, Kristina Ehrhardt, Neha Kashyap, Michael A. White, Leonidas Bleris
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10.1371/journal.pone.0120396

PLOS ONE Large Genomic Fragment Deletions and Insertions in Mouse Using CRISPR/Cas9
March 24, 2015 Luqing Zhang, Ruirui Jia, Norberto J. Palange, Achim Cchitvsanzwhoh Satheka, Jacques Togo, Yao An, Mabwi Humphrey, Luying Ban, Yan Ji, Honghong Jin, Xuechao Feng, Yaowu Zheng
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10.1371/journal.pone.0136930

PLOS ONE Generation of SNCA Cell Models Using Zinc Finger Nuclease (ZFN) Technology for Efficient High-Throughput Drug Screening
August 28, 2015 Warunee Dansithong, Sharan Paul, Daniel R. Scoles, Stefan M. Pulst, Duong P. Huynh
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10.1371/journal.pone.0129457

PLOS ONE Pervasive Genotypic Mosaicism in Founder Mice Derived from Genome Editing through Pronuclear Injection
June 8, 2015 Daniel Oliver, Shuiqiao Yuan, Hayden McSwiggin, Wei Yan

High Throughput Sequencing

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10.1371/journal.pone.0154038

PLOS ONE Validation and Application of a Custom-Designed Targeted Next-Generation Sequencing Panel for the Diagnostic Mutational Profiling of Solid Tumors
April 21, 2016 Guy Froyen, An Broekmans, Femke Hillen, Karin Pat, Ruth Achten, Jeroen Mebis, Jean-Luc Rummens, Johan Willemse, Brigitte Maes
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10.1371/journal.pone.0127376

PLOS ONE C9ORF72 GGGGCC Expanded Repeats Produce Splicing Dysregulation which Correlates with Disease Severity in Amyotrophic Lateral Sclerosis
May 27, 2015 Johnathan Cooper-Knock, Joanna J. Bury, Paul R Heath, Matthew Wyles, Adrian Higginbottom, Catherine Gelsthorpe, J. Robin Highley, Guillaume Hautbergue, Magnus Rattray, Janine Kirby, Pamela J. Shaw
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10.1371/journal.pone.0152673

PLOS ONE Colorectal Cancer Genetic Heterogeneity Delineated by Multi-Region Sequencing
March 29, 2016 You-Wang Lu, Hui-Feng Zhang, Rui Liang, Zhen-Rong Xie, Hua-You Luo, Yu-Jian Zeng, Yu Xu, La-Mei Wang, Xiang-Yang Kong, Kun-Hua Wang
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10.1371/journal.pone.0159232

PLOS ONE Microsatellite Length Scoring by Single Molecule Real Time Sequencing – Effects of Sequence Structure and PCR Regime
July 14, 2016 Mikkel Meyn Liljegren, Eric Jacques de Muinck, Pål Trosvik

Cell Signalling in Exercise & Metabolic Dysfunction

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10.1371/journal.pone.0127089

PLOS ONE Gene and MicroRNA Expression Responses to Exercise; Relationship with Insulin Sensitivity
May 18, 2015 Carrie S. McLean, Clinton Mielke, Jeanine M. Cordova, Paul R. Langlais, Benjamin Bowen, Danielle Miranda, Dawn K. Coletta, Lawrence J. Mandarino
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10.1371/journal.pone.0145836

PLOS ONE Mitochondrial Dysfunction Plus High-Sugar Diet Provokes a Metabolic Crisis That Inhibits Growth
January 26, 2016 Esko Kemppainen, Jack George, Görkem Garipler, Tea Tuomela, Essi Kiviranta, Tomoyoshi Soga, Cory D. Dunn, Howard T. Jacobs
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10.1371/journal.pone.0122982

PLOS ONE Abnormalities of AMPK Activation and Glucose Uptake in Cultured Skeletal Muscle Cells from Individuals with Chronic Fatigue Syndrome
April 2, 2015 Audrey E. Brown, David E. Jones, Mark Walker, Julia L. Newton
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10.1371/journal.pone.0123881

PLOS ONE The Single Nucleotide Polymorphism Gly482Ser in the PGC-1α Gene Impairs Exercise-Induced Slow-Twitch Muscle Fibre Transformation in Humans
April 17, 2015 Peter Steinbacher, René G. Feichtinger, Lyudmyla Kedenko, Igor Kedenko, Sandra Reinhardt, Anna-Lena Schönauer, Isabella Leitner, Alexandra M. Sänger, Walter Stoiber, Barbara Kofler, Holger Förster, Bernhard Paulweber, Susanne Ring-Dimitriou
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10.1371/journal.pone.0129647

PLOS ONE Epigenetic Modifications of the PGC-1α Promoter during Exercise Induced Expression in Mice
June 8, 2015 Timothy L. Lochmann, Ravindar R. Thomas, James P. Bennett Jr, Shirley M. Taylor

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