Biology & Life Sciences

Synthetic Biology: Biological Parts

Published January 31, 2017 Curated Collections

Synthetic Biology is an emerging, trans-disciplinary field at the intersection between many engineering and scientific disciplines such as biology, chemical engineering, chemistry, electrical engineering, and computer science. This section of the Synthetic Biology Collection details papers related to biological parts.

Image Credit: Ivan Morozov (Virginia Bioinformatics Institute) / PLOS

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Research Articles

Research Articles

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10.1371/journal.pone.0158447

PLOS ONE Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications
July 5, 2016 Yafeng Song, Jonas Nikoloff, Gang Fu, Jingqi Chen, Qinggang Li, Nengzhong Xie, Ping Zheng, Jibin Sun, Dawei Zhang
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10.1371/journal.pone.0155177

PLOS ONE Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors in Embryonic Stem Cells
May 6, 2016 Hanna Sladitschek, Pierre Neveu
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10.1371/journal.pone.0154759

PLOS ONE Enhanced snoMEN Vectors Facilitate Establishment of GFP–HIF-1α Protein Replacement Human Cell Lines
April 29, 2016 Motoharu Ono, Kayo Yamada, Dalila Bensaddek, Vackar Afzal, John Biddlestone, Brian Ortmann, Sharon Mudie, Vincent Boivin, Michelle S. Scott, Sonia Rocha, Angus I. Lamond
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10.1371/journal.pone.0152146

PLOS ONE Engineering Bacteria to Search for Specific Concentrations of Molecules by a Systematic Synthetic Biology Design Method
April 20, 2016 Shin-Ming Tien, Chih-Yuan Hsu, Bor-Sen Chen
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10.1371/journal.ppat.1005502

PLOS Pathogens A Functional Bacterium-to-Plant DNA Transfer Machinery of Rhizobium etli
March 11, 2016 Benoît Lacroix, Vitaly Citovsky
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10.1371/journal.pone.0150182

PLOS ONE Reproducibility of Fluorescent Expression from Engineered Biological Constructs in E. coli
March 3, 2016 Jacob Beal, Traci Haddock-Angelli, Markus Gershater, Kim de Mora, Meagan Lizarazo, Jim Hollenhorst, Randy Rettberg
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PLOS

PLOS Synbio Community Related Blog: Student teams take on synbio reproducibility problem
November 18, 2016 Aaron Dy
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10.1371/journal.pone.0149746

PLOS ONE N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli
February 22, 2016 Karthik Sekar, Andrew M. Gentile, John W. Bostick, Keith E. J. Tyo
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10.1371/journal.pone.0148320

PLOS ONE Synthetic Transcription Amplifier System for Orthogonal Control of Gene Expression in Saccharomyces cerevisiae
February 22, 2016 Anssi Rantasalo, Elena Czeizler, Riitta Virtanen, Juho Rousu, Harri Lähdesmäki, Merja Penttilä, Jussi Jäntti, Dominik Mojzita
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10.1371/journal.pone.0149483

PLOS ONE Modular Synthetic Inverters from Zinc Finger Proteins and Small RNAs
February 17, 2016 Justin Hsia, William J. Holtz, Michel M. Maharbiz, Murat Arcak, Jay D. Keasling
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10.1371/journal.pone.0148918

PLOS ONE Engineered Promoters for Potent Transient Overexpression
February 12, 2016 Dan Even, Adi Kedmi, Shani Basch-Barzilay, Diana Ideses, Ravid Tikotzki, Hila Shir-Shapira, Orit Shefi, Tamar Juven-Gershon
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10.1371/journal.pone.0005553

PLOS ONE Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes
May 14, 2009 Carola Engler, Ramona Gruetzner, Romy Kandzia, Sylvestre Marillonnet
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10.1371/journal.pone.0006441

PLOS ONE Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
July 30, 2009 Jiayuan Quan, Jingdong Tian
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10.1371/journal.pone.0011024

PLOS ONE DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis
June 11, 2010 Michael A. Jensen, Marilyn Fukushima, Ronald W. Davis
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10.1371/journal.pone.0019722

PLOS ONE Assembly of Designer TAL Effectors by Golden Gate Cloning
May 19, 2011 Ernst Weber, Ramona Gruetzner, Stefan Werner, Carola Engler, Sylvestre Marillonnet
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10.1371/journal.pone.0016765

PLOS ONE A Modular Cloning System for Standardized Assembly of Multigene Constructs
February 18, 2011 Ernst Weber, Carola Engler, Ramona Gruetzner, Stefan Werner, Sylvestre Marillonnet
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10.1371/journal.pone.0021622

PLOS ONE GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules
July 7, 2011 Alejandro Sarrion-Perdigones, Erica Falconi, Sara Zandalinas, Paloma Juárez, Asun Fernández-del-Carmen, Antonio Granell, Diego Orzaez
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10.1371/journal.pone.0024906

PLOS ONE Microarray Generation of Thousand-Member Oligonucleotide Libraries
September 23, 2011 Nina Svensen, Juan José Díaz-Mochón, Mark Bradley
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10.1371/journal.pone.0026222

PLOS ONE OmniChange: The Sequence Independent Method for Simultaneous Site-Saturation of Five Codons
October 19, 2011 Alexander Dennig, Amol Shivange, Jan Marienhagen, Ulrich Schwaneberg
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10.1371/journal.pone.0027062

PLOS ONE Chemical Synthesis of Bacteriophage G4
November 16, 2011 Ruilin Yang, Yonghua Han, Yiwang Ye, Zhimao Jiang, Yuchen Liu, Yaoting Gui, Zhiming Cai
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10.1371/journal.pone.0030267

PLOS ONE In Vitro Assembly of Multiple DNA Fragments Using Successive Hybridization
January 26, 2012 Xinglin Jiang, Jianming Yang, Haibo Zhang, Huibin Zou, Cong Wang, Mo Xian
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10.1371/journal.pone.0056854

PLOS ONE Parallel In Vivo DNA Assembly by Recombination: Experimental Demonstration and Theoretical Approaches
February 28, 2013 Zhenyu Shi, Anthony Wedd, Sally Gras
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10.1371/journal.pone.0057943

PLOS ONE DNA Fragments Assembly Based on Nicking Enzyme System
March 6, 2013 Rui-Yan Wang, Zhen-Yu Shi, Ying-Ying Guo, Jin-Chun Chen, Guo-Qiang Chen
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10.1371/journal.pone.0107329

PLOS ONE A Rapid and Simple Method for DNA Engineering Using Cycled Ligation Assembly
September 16, 2014 Theodore Roth, Ljiljana Milenkovic, Matthew Scott
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© Vazquez-Vilar et al. 2016

BMC Plant Methods A modular toolbox for gRNA–Cas9 genome engineering in plants based on the GoldenBraid standard
September 15, 2015 Marta Vazquez-Vilar, Joan Miquel Bernabé-Orts, Asun Fernandez-del-Carmen et al.
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Gavrilovic et al. 2016

BMC Plant Methods Inoculation insensitive promoters for cell type enriched gene expression in legume roots and nodules
January 22, 2016 Srdjan Gavrilovic, Zhe Yan, Anna M. Jurkiewicz, Jens Stougaard & Katharina Markmann

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