Biology & Life Sciences

Synthetic Biology: DNA Synthesis & Assembly

Published January 30, 2017 Curated Collections

Synthetic Biology is an emerging, trans-disciplinary field at the intersection between many engineering and scientific disciplines such as biology, chemical engineering, chemistry, electrical engineering, and computer science. This section of the Synthetic Biology Collection details papers related to DNA Synthesis & Assembly.

Image Credit: Ivan Morozov (Virginia Bioinformatics Institute) / PLOS

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Research Articles

Research Articles

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10.1371/journal.pone.0005553

PLOS ONE Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes
May 14, 2009 Carola Engler, Ramona Gruetzner, Romy Kandzia, Sylvestre Marillonnet
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10.1371/journal.pone.0006441

PLOS ONE Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
July 30, 2009 Jiayuan Quan, Jingdong Tian
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10.1371/journal.pone.0011024

PLOS ONE DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis
June 11, 2010 Michael A. Jensen, Marilyn Fukushima, Ronald W. Davis
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10.1371/journal.pone.0016765

PLOS ONE A Modular Cloning System for Standardized Assembly of Multigene Constructs
February 18, 2011 Ernst Weber, Carola Engler, Ramona Gruetzner, Stefan Werner, Sylvestre Marillonnet
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10.1371/journal.pone.0019722

PLOS ONE Assembly of Designer TAL Effectors by Golden Gate Cloning
May 19, 2011 Ernst Weber, Ramona Gruetzner, Stefan Werner, Carola Engler, Sylvestre Marillonnet
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10.1371/journal.pone.0021622

PLOS ONE GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules
July 7, 2011 Alejandro Sarrion-Perdigones, Erica Falconi, Sara Zandalinas, Paloma Juárez, Asun Fernández-del-Carmen, Antonio Granell, Diego Orzaez
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10.1371/journal.pone.0026222

PLOS ONE OmniChange: The Sequence Independent Method for Simultaneous Site-Saturation of Five Codons
October 19, 2011 Alexander Dennig, Amol Shivange, Jan Marienhagen, Ulrich Schwaneberg
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10.1371/journal.pone.0024906

PLOS ONE Microarray Generation of Thousand-Member Oligonucleotide Libraries
September 23, 2011 Nina Svensen, Juan José Díaz-Mochón, Mark Bradley
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10.1371/journal.pone.0027062

PLOS ONE Chemical Synthesis of Bacteriophage G4
November 16, 2011 Ruilin Yang, Yonghua Han, Yiwang Ye, Zhimao Jiang, Yuchen Liu, Yaoting Gui, Zhiming Cai
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10.1371/journal.pone.0030267

PLOS ONE In Vitro Assembly of Multiple DNA Fragments Using Successive Hybridization
January 26, 2012 Xinglin Jiang, Jianming Yang, Haibo Zhang, Huibin Zou, Cong Wang, Mo Xian
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10.1371/journal.pone.0056854

PLOS ONE Parallel In Vivo DNA Assembly by Recombination: Experimental Demonstration and Theoretical Approaches
February 28, 2013 Zhenyu Shi, Anthony Wedd, Sally Gras
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10.1371/journal.pone.0057943

PLOS ONE DNA Fragments Assembly Based on Nicking Enzyme System
March 6, 2013 Rui-Yan Wang, Zhen-Yu Shi, Ying-Ying Guo, Jin-Chun Chen, Guo-Qiang Chen
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10.1371/journal.pone.0107329

PLOS ONE A Rapid and Simple Method for DNA Engineering Using Cycled Ligation Assembly
September 16, 2014 Theodore Roth, Ljiljana Milenkovic, Matthew Scott

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