Biology & Life Sciences

Synthetic Biology: Genome Editing

Published January 30, 2017 Curated Collections

Synthetic Biology is an emerging, trans-disciplinary field at the intersection between many engineering and scientific disciplines such as biology, chemical engineering, chemistry, electrical engineering, and computer science. This section of the Synthetic Biology Collection details papers related to Genome Editing.

Image Credit: Ivan Morozov (Virginia Bioinformatics Institute) / PLOS

Jump to section

CRISPR
Talens
Zinc Finger Nucleases
Other

CRISPR

Image credit
10.1371/journal.pone.0158294

PLOS ONE Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional Activator Systems
June 24, 2016 Julia K. Bialek, Gábor A. Dunay, Maike Voges, Carola Schäfer, Michael Spohn, Rolf Stucka, Joachim Hauber, Ulrike C. Lange
Image credit
10.1371/journal.ppat.1005701

PLOS Pathogens CRISPR/Cas9-Mediated Genome Editing of Herpesviruses Limits Productive and Latent Infections
June 30, 2016 Ferdy R. van Diemen, Elisabeth M. Kruse, Marjolein J. G. Hooykaas, Carlijn E. Bruggeling, Anita C. Schürch, Petra M. van Ham, Saskia M. Imhof, Monique Nijhuis, Emmanuel J. H. J. Wiertz, Robert Jan Lebbink
Image credit
10.1371/journal.pone.0144970

PLOS ONE Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions
January 20, 2016 Yi Li, Saurabh Mendiratta, Kristina Ehrhardt, Neha Kashyap, Michael A. White, Leonidas Bleris
Image credit
10.1371/journal.pone.0148362

PLOS ONE A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs
February 5, 2016 Peter J. Romanienko, Joseph Giacalone, Joanne Ingenito, Yijie Wang, Mayumi Isaka, Thomas Johnson, Yun You, Willie H. Mark
Image credit
10.1371/journal.pcbi.1004724

PLOS Computational Biology A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation
January 29, 2016 Iman Farasat, Howard M. Salis
Image credit
10.1371/journal.pone.0095101

PLOS ONE CRISPR/Cas9 Allows Efficient and Complete Knock-In of a Destabilization Domain-Tagged Essential Protein in a Human Cell Line, Allowing Rapid Knockdown of Protein Function
April 17, 2014 Arnold Park, Sohui Won, Mickey Pentecost, Wojciech Bartkowski, Benhur Lee
Image credit
10.1371/journal.pone.0089413

PLOS ONE Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9
March 5, 2014 Yuanwu Ma, Bin Shen, Xu Zhang, Yingdong Lu, Wei Chen, Jing Ma, Xingxu Huang, lianfeng zhang
Image credit
10.1371/journal.pone.0093806

PLOS ONE Targeted Genome Editing of Sweet Orange Using Cas9/sgRNA
April 7, 2014 Hongge Jia, Nian Wang
Image credit
10.1371/journal.pone.0068708

PLOS ONE Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System
July 9, 2013 Woong Hwang, Yanfang Fu, Deepak Reyon, Morgan Maeder, Prakriti Kaini, Jeffry Sander, J. Joung, Randall Peterson, Jing-Ruey Yeh

Talens

Image credit
10.1371/journal.pone.0096114

PLOS ONE Simple and Efficient Methods for Enrichment and Isolation of Endonuclease Modified Cells
May 5, 2014 Branden Moriarity, Eric rahrmann, Dominic Beckmann, Caitlin Conboy, Adrienne Watson, Dan Carlson, Erik Olson, kendra Hyland, scott Fahrenkrug, R. Scott McIvor, David Largaespada
Image credit
10.1371/journal.pone.0096483

PLOS ONE The Position of DNA Cleavage by TALENs and Cell Synchronization Influences the Frequency of Gene Editing Directed by Single-Stranded Oligonucleotides
May 1, 2014 Natalia Rivera-Torres, Bryan Strouse, Pawel Bialk, Rohina Niamat, Eric Kmiec
Image credit
10.1371/journal.pone.0095790

PLOS ONE Activation of Silenced Cytokine Gene Promoters by the Synergistic Effect of TBP-TALE and VP64-TALE Activators
April 22, 2014 Kim Anthony, Abhijit More, Xiaoliu Zhang
Image credit
10.1371/journal.pone.0093575

PLOS ONE Precise Gene Modification Mediated by TALEN and Single-Stranded Oligodeoxynucleotides in Human Cells
April 1, 2014 Xiaoling Wang, Yingjia Wang, He Huang, Buyuan Chen, Xinji Chen, Jianda Hu, Tammy Chang, Ren-Jang Lin, Jiing-Kuan Yee
Image credit
10.1371/journal.pone.0095611

PLOS ONE Highly Specific Contractions of a Single CAG/CTG Trinucleotide Repeat by TALEN in Yeast
April 18, 2014 Guy-Franck Richard, David Viterbo, Varun Khanna, Valentine Mosbach, Lauriane Castelain, Bernard Dujon
Image credit
10.1371/journal.pone.0092046

PLOS ONE True-Breeding Targeted Gene Knock-Out in Barley Using Designer TALE-Nuclease in Haploid Cells
March 18, 2014 Maia Gurushidze, Goetz Hensel, Stefan Hiekel, Sindy Schedel, Vladimir Valkov, Jochen Kumlehn
Image credit
10.1371/journal.pone.0091547

PLOS ONE Bacterial Delivery of TALEN Proteins for Human Genome Editing
March 11, 2014 Jingyue Jia, Yongxin Jin, Ting Bian, Donghai Wu, Lijun Yang, Naohiro Terada, Weihui Wu, Shouguang Jin
Image credit
10.1371/journal.pone.0089631

PLOS ONE TALEN-Mediated Modification of the Bovine Genome for Large-Scale Production of Human Serum Albumin
February 21, 2014 Shaida Moghaddassi, Will Eyestone, Colin Bishop
Image credit
10.1371/journal.pone.0088146

PLOS ONE Generation of TALEN-Mediated GR^dim Knock-In Rats by Homologous Recombination
February 11, 2014 Veronica Ponce de Leon, Anne Mérillat, Laurent Tesson, Ignacio Anegón, Edith Hummler
Image credit
10.1371/journal.pone.0088124

PLOS ONE Establishment of HeLa Cell Mutants Deficient in Sphingolipid-Related Genes Using TALENs
February 3, 2014 Toshiyuki Yamaji, Kentaro Hanada
Image credit
10.1371/journal.pone.0084893

PLOS ONE A Modified TALEN-Based Strategy for Rapidly and Efficiently Generating Knockout Mice for Kidney Development Studies
January 8, 2014 Yunhong Liu, Xiaoyan Lv, Ruizhi Tan, Tianming Liu, Tielin Chen, Mi Li, Yuhang Liu, Fang Nie, Xiaoyan Wang, Puhui Zhou, Mianzhi Chen, Qin Zhou
Image credit
10.1371/journal.pone.0085755

PLOS ONE Cell-Penetrating Peptide-Mediated Delivery of TALEN Proteins via Bioconjugation for Genome Engineering
January 20, 2014 Jia Liu, Thomas Gaj, James Patterson, Shannon Sirk, Carlos Barbas
Image credit
10.1371/journal.pone.0084176

PLOS ONE Tandem Repeat Modification during Double-Strand Break Repair Induced by an Engineered TAL Effector Nuclease in Zebrafish Genome
December 26, 2013 Wanxu Huang, Jianbo Zheng, Ying He, Chen Luo
Image credit
10.1371/journal.pone.0084250

PLOS ONE Highly Efficient Generation of GGTA1 Biallelic Knockout Inbred Mini-Pigs with TALENs
December 17, 2013 Jige Xin, Huaqiang Yang, Nana Fan, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Xiaoping Li, Jun Song, Yi Yang, Qingjian Zou, Quanmei Yan, Yangzhi Zeng, Liangxue Lai
Image credit
10.1371/journal.pone.0082539

PLOS ONE TALE-PvuII Fusion Proteins – Novel Tools for Gene Targeting
December 5, 2013 Mert Yanik, Jamal Alzubi, Thomas Lahaye, Toni Cathomen, Alfred Pingoud, Wolfgang Wende
Image credit
10.1371/journal.pone.0078678

PLOS ONE Targeted Gene Therapy of Xeroderma Pigmentosum Cells Using Meganuclease and TALEN™
November 13, 2013 Aurélie Dupuy, Julien Valton, Sophie Leduc, Jacques Armier, Roman Galetto, Agnès Gouble, Céline Lebuhotel, Anne Stary, Frédéric Pâques, Phillipe Duchateau, Alain Sarasin, Fayza Daboussi
Image credit
10.1371/journal.pone.0080281

PLOS ONE Rapid Assembly of Customized TALENs into Multiple Delivery Systems
November 7, 2013 Zhengxing Zhang, Siliang Zhang, Xin Huang, Kyle Orwig, Yi Sheng
Image credit
10.1371/journal.pone.0076004

PLOS ONE Targeted Gene Deletion of miRNAs in Mice by TALEN System
October 16, 2013 Shuji Takada, Tempei Sato, Yoshiaki Ito, Satoshi Yamashita, Tomoko Kato, Miyuri Kawasumi, Masami Kanai-Azuma, Arisa Igarashi, Tomomi Kato, Moe Tamano, Hiroshi Asahara
Image credit
10.1371/journal.pone.0076387

PLOS ONE Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes in Zebrafish Using TALENs
October 10, 2013 Yun Liu, Daji Luo, Hui Zhao, Zuoyan Zhu, Wei Hu, Christopher Cheng
Image credit
10.1371/journal.pone.0076045

PLOS ONE Molecular Dynamics Simulations of DNA-Free and DNA-Bound TAL Effectors
October 10, 2013 Hua Wan, Jian-ping Hu, Kang-shun Li, Xu-hong Tian, Shan Chang
Image credit
10.1371/journal.pone.0073458

PLOS ONE Efficient TALEN Construction for Bombyx mori Gene Targeting
September 18, 2013 Yoko Takasu, Suresh Sajwan, Takaaki Daimon, Mizuko Osanai-Futahashi, Keiro Uchino, Hideki Sezutsu, Toshiki Tamura, Michal Zurovec
Image credit
10.1371/journal.pone.0074511

PLOS ONE Targeted Mutagenesis in the Malaria Mosquito Using TALE Nucleases
August 15, 2013 Andrea Smidler, Olivier Terenzi, Julien Soichot, Elena Levashina, Eric Marois
Image credit
10.1371/journal.pone.0066459

PLOS ONE A Simple and Efficient Method for Assembling TALE Protein Based on Plasmid Library
June 20, 2013 Zhiqiang Zhang, Duo Li, Huarong Xu, Ying Xin, Tingting Zhang, Lixia Ma, Xin Wang, Zhilong Chen, zhiying zhang
Image credit
10.1371/journal.pone.0065259

PLOS ONE High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD GoldyTALEN Design
May 29, 2013 Alvin Ma, Han Lee, Karl Clark, Stephen Ekker
Image credit
10.1371/journal.pone.0063074

PLOS ONE Targeting Human MicroRNA Genes Using Engineered Tal-Effector Nucleases (TALENs)
May 7, 2013 Ruozhen Hu, Jared Wallace, Timothy Dahlem, David Grunwald, Ryan O'connell
Image credit
10.1371/journal.pone.0060082

PLOS ONE TALEN-Based Gene Disruption in the Dengue Vector Aedes aegypti
March 21, 2013 Azadeh Aryan, Michelle Anderson, Kevin Myles, Zach Adelman
Image credit
10.1371/journal.pone.0060216

PLOS ONE Site Specific Mutation of the Zic2 Locus by Microinjection of TALEN mRNA in Mouse CD1, C3H and C57BL/6J Oocytes
March 28, 2013 Benjamin Davies, Graham Davies, Christopher Preece, Rathi Puliyadi, Dorota Szumska, Shoumo Bhattacharya
Image credit
10.1371/journal.pone.0045383

PLOS ONE Targeting G with TAL Effectors: A Comparison of Activities of TALENs Constructed with NN and NK Repeat Variable Di-Residues
September 24, 2012 Michelle Christian, Zachary Demorest, Colby Starker, Mark Osborn, Michael Nyquist, Yong Zhang, Daniel Carlson, Philip Bradley, Adam Bogdanove, Daniel Voytas
Image credit
10.1371/journal.pone.0037877

PLOS ONE Improved Somatic Mutagenesis in Zebrafish Using Transcription Activator-Like Effector Nucleases (TALENs)
May 24, 2012 Finola Moore, Deepak Reyon, Jeffry Sander, Sarah Martinez, Jessica Blackburn, Cyd Khayter, Cherie Ramirez, J. Joung, David Langenau
Image credit
10.1371/journal.pone.0045035

PLOS ONE Highly Efficient and Specific Genome Editing in Silkworm Using Custom TALENs
September 18, 2012 Sanyuan Ma, Shengling Zhang, Feng Wang, Yong Liu, Yuanyuan Liu, Hanfu Xu, Chun Liu, Ying Lin, Ping Zhao, Qingyou Xia

Zinc Finger Nucleases

Image credit
10.1371/journal.pone.0097579

PLOS ONE AAV-Mediated Delivery of Zinc Finger Nucleases Targeting Hepatitis B Virus Inhibits Active Replication
May 14, 2014 Nicholas Weber, Daniel Stone, Ruth Sedlak, Harshana De Silva Feelixge, Pavitra Roychoudhury, Joshua Schiffer, Martine Aubert, Keith R Jerome
Image credit
10.1371/journal.pone.0085650

PLOS ONE A Novel Genetic System Based on Zinc Finger Nucleases for the Identification of Interactions between Proteins In Vivo
December 31, 2013 Ling Wang, Kun Xu, Juan Lin, Simin Shao, Tingting Zhang, Huarong Xu, Zehui Wei, zhiying zhang
Image credit
10.1371/journal.pone.0081131

PLOS ONE Targeting of Herpes Simplex Virus 1 Thymidine Kinase Gene Sequences into the OCT4 Locus of Human Induced Pluripotent Stem Cells
November 29, 2013 Wu Ou, Pingjuan Li, Jakob Reiser
Image credit
10.1371/journal.pone.0078839

PLOS ONE Functional Analysis of a Breast Cancer-Associated FGFR2 Single Nucleotide Polymorphism Using Zinc Finger Mediated Genome Editing
November 12, 2013 Luisa Robbez-Masson, Csaba Bödör, J. Louise Jones, Helen Hurst, Jude Fitzgibbon, Ian Hart, Richard Grose
Image credit
10.1371/journal.pone.0075493

PLOS ONE An Over Expression APP Model for Anti-Alzheimer Disease Drug Screening Created by Zinc Finger Nuclease Technology
November 6, 2013 Xiaojing Zhang, Huanran Tan, Hui Li, Yiqing Mao, Zhixin Li, Rong Wang, Tingting Guo, Ling Jin, Rongjing Song, Wei Xu, Na Zhou, Yizhuang Zhang, Ruobi Hu, Xi Wang, Huakang Huang, Zhen Lei, Gang Niu, David Irwin
Image credit
10.1371/journal.pone.0077696

PLOS ONE Efficient Knockin Mouse Generation by ssDNA Oligonucleotides and Zinc-Finger Nuclease Assisted Homologous Recombination in Zygotes
October 22, 2013 Bin Shen, Xin Zhang, Yinan Du, Jianying Wang, Jun Gong, Xiaodong Zhang, Peri Tate, Hongliang Li, Xingxu Huang, Wensheng Zhang
Image credit
10.1371/journal.pone.0076478

PLOS ONE Generation of Interleukin-2 Receptor Gamma Gene Knockout Pigs from Somatic Cells Genetically Modified by Zinc Finger Nuclease-Encoding mRNA
October 9, 2013 Masahito Watanabe, Kazuaki Nakano, Hitomi Matsunari, Taisuke Matsuda, Miki Maehara, Takahiro Kanai, Mirina Kobayashi, Yukina Matsumura, Rieko Sakai, Momoko Kuramoto, Gota Hayashida, Yoshinori Asano, Shuko Takayanagi, Yoshikazu Arai, Kazuhiro Umeyama, Masaki Nagaya, Yutaka Hanazono, Hiroshi Nagashima
Image credit
10.1371/journal.pone.0068391

PLOS ONE Tagging of Genomic STAT3 and STAT1 with Fluorescent Proteins and Insertion of a Luciferase Reporter in the Cyclin D1 Gene Provides a Modified A549 Cell Line to Screen for Selective STAT3 Inhibitors
July 9, 2013 Andrey Samsonov, Nathan Zenser, Fan Zhang, Hongyi Zhang, John Fetter, Dmitry Malkov
Image credit
10.1371/journal.pone.0065267

PLOS ONE Zinc Finger Nuclease Mediated Knockout of ADP-Dependent Glucokinase in Cancer Cell Lines: Effects on Cell Survival and Mitochondrial Oxidative Metabolism
June 14, 2013 Susan Richter, Shona Morrison, Tim Connor, Jiechuang Su, Cristin Print, Ron Ronimus, Sean McGee, William Wilson
Image credit
10.1371/journal.pone.0064687

PLOS ONE Simultaneous Screening and Validation of Effective Zinc Finger Nucleases in Yeast
May 31, 2013 Ling Wang, Juan Lin, Tingting Zhang, Kun Xu, Chonghua Ren, zhiying zhang
Image credit
10.1371/journal.pone.0059801

PLOS ONE Repeatable Construction Method for Engineered Zinc Finger Nuclease Based on Overlap Extension PCR and TA-Cloning
March 25, 2013 Wataru Fujii, Kiyoshi Kano, Koji Sugiura, Kunihiko Naito
Image credit
10.1371/journal.pone.0057239

PLOS ONE Efficient Methods for Targeted Mutagenesis in Zebrafish Using Zinc-Finger Nucleases: Data from Targeting of Nine Genes Using CompoZr or CoDA ZFNs
February 22, 2013 Raman Sood, Blake Carrington, Kevin Bishop, MaryPat Jones, Alberto Rissone, Fabio Candotti, Settara Chandrasekharappa, Paul Liu
Image credit
10.1371/journal.pone.0056476

PLOS ONE Magnetic Separation and Antibiotics Selection Enable Enrichment of Cells with ZFN/TALEN-Induced Mutations
February 18, 2013 Hyojin Kim, Myung-Sun Kim, Gabbine Wee, Choong-il Lee, Hyongbum Kim, Jin-Soo Kim
Image credit
10.1371/journal.pone.0056633

PLOS ONE Gene- and Protein-Delivered Zinc Finger–Staphylococcal Nuclease Hybrid for Inhibition of DNA Replication of Human Papillomavirus
February 20, 2013 Takashi Mino, Tomoaki Mori, Yasuhiro Aoyama, Takashi Sera
Image credit
10.1371/journal.pone.0046971

PLOS ONE Targeted Genomic Integration of a Selectable Floxed Dual Fluorescence Reporter in Human Embryonic Stem Cells
October 10, 2012 Jay Gantz, Nathan Palpant, Robert Welikson, Stephen Hauschka, Charles Murry, Michael Laflamme
Image credit
10.1371/journal.pone.0054282

PLOS ONE Stability of Zinc Finger Nuclease Protein Is Enhanced by the Proteasome Inhibitor MG132
January 24, 2013 Suresh Ramakrishna, Young-Hoon Kim, Hyongbum Kim
Image credit
10.1371/journal.pone.0045169

PLOS ONE Rescue the Failed Half-ZFN by a Sensitive Mammalian Cell-Based Luciferase Reporter System
September 18, 2012 Weifeng Zhang, Yuanxu Guo, Chen Zhang, Haiyan Ji, Wenpeng Meng, Dongyang Wang, Xing Li, Qinwen Mao, Haibin Xia
Image credit
10.1371/journal.pone.0043968

PLOS ONE Zebrafish foxP2 Zinc Finger Nuclease Mutant Has Normal Axon Pathfinding
August 24, 2012 Lingyan Xing, Kazuyuki Hoshijima, David Grunwald, Esther Fujimoto, Tyler Quist, Jacob Sneddon, Chi-Bin Chien, Tamara Stevenson, Joshua Bonkowsky
Image credit
10.1371/journal.pone.0041796

PLOS ONE Evaluation of OPEN Zinc Finger Nucleases for Direct Gene Targeting of the ROSA26 Locus in Mouse Embryos
September 6, 2012 Mario Hermann, Morgan Maeder, Kyle Rector, Joseph Ruiz, Burkhard Becher, Kurt Bürki, Cyd Khayter, Adriano Aguzzi, J. Joung, Thorsten Buch, Pawel Pelczar
Image credit
10.1371/journal.pone.0028911

PLOS ONE Selection-Independent Generation of Gene Knockout Mouse Embryonic Stem Cells Using Zinc-Finger Nucleases
December 14, 2011 Anna Osiak, Frank Radecke, Eva Guhl, Sarah Radecke, Nadine Dannemann, Fabienne Lütge, Silke Glage, Cornelia Rudolph, Tobias Cantz, Klaus Schwarz, Regine Heilbronn, Toni Cathomen
Image credit
10.1371/journal.pone.0028897

PLOS ONE Heritable Targeted Inactivation of Myostatin Gene in Yellow Catfish (Pelteobagrus fulvidraco) Using Engineered Zinc Finger Nucleases
December 14, 2011 Zhangji Dong, Jiachun Ge, Kui Li, Zhiqiang Xu, Dong Liang, Jingyun Li, Junbo Li, Wenshuang Jia, Yuehua Li, Xiaohua Dong, Shasha Cao, Xiaoxiao Wang, Jianlin Pan, Qingshun Zhao
Image credit
10.1371/journal.pone.0021045

PLOS ONE Efficient Immunoglobulin Gene Disruption and Targeted Replacement in Rabbit Using Zinc Finger Nucleases
June 13, 2011 Tatiana Flisikowska, Irmgard Thorey, Sonja Offner, Francesca Ros, Valeria Lifke, Bryan Zeitler, Oswald Rottmann, Anna Vincent, Lei Zhang, Shirin Jenkins, Helmut Niersbach, Alexander Kind, Philip Gregory, Angelika Schnieke, Josef Platzer
Image credit
10.1371/journal.pone.0020913

PLOS ONE p53 Gene Repair with Zinc Finger Nucleases Optimised by Yeast 1-Hybrid and Validated by Solexa Sequencing
June 9, 2011 Frank Herrmann, Mireia Garriga-Canut, Rebecca Baumstark, Emmanuel Fajardo-Sanchez, James Cotterell, André Minoche, Heinz Himmelbauer, Mark Isalan
Image credit
10.1371/journal.pone.0020514

PLOS ONE Repair at Single Targeted DNA Double-Strand Breaks in Pluripotent and Differentiated Human Cells
May 25, 2011 Hua Fung, David Weinstock
Image credit
10.1371/journal.pone.0016961

PLOS ONE Dissection of Splicing Regulation at an Endogenous Locus by Zinc-Finger Nuclease-Mediated Gene Editing
February 8, 2011 Sandra Cristea, Philip Gregory, Fyodor Urnov, Gregory Cost
Image credit
10.1371/journal.pone.0008870

PLOS ONE Generation of Knockout Rats with X-Linked Severe Combined Immunodeficiency (X-SCID) Using Zinc-Finger Nucleases
January 25, 2010 Tomoji Mashimo, Akiko Takizawa, Birger Voigt, Kazuto Yoshimi, Hiroshi Hiai, Takashi Kuramoto, Tadao Serikawa
Image credit
10.1371/journal.pone.0004348

PLOS ONE Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)
February 9, 2009 Jonathan E. Foley, Jing-Ruey J. Yeh, Morgan L. Maeder, Deepak Reyon, Jeffry D. Sander, Randall T. Peterson, J. Keith Joung
Image credit
10.1371/journal.pone.0095225

PLOS ONE Efficient Generation of Myostatin (MSTN) Biallelic Mutations in Cattle Using Zinc Finger Nucleases
April 17, 2014 Junjie Luo, Zhiyuan Song, Shengli Yu, Dan Cui, Benli Wang, FangRong Ding, Song Li, Yunping Dai, Ning Li

Other

Image credit
10.1371/journal.ppat.1005502

PLOS Pathogens A Functional Bacterium-to-Plant DNA Transfer Machinery of Rhizobium etli
March 11, 2016 Benoît Lacroix, Vitaly Citovsky
Image credit
10.1371/journal.pgen.1005926

PLOS Genetics The Selective Advantage of Synonymous Codon Usage Bias in Salmonella
March 10, 2016 Gerrit Brandis, Diarmaid Hughes
Image credit
10.1371/journal.pone.0088840

PLOS ONE To Nick or Not to Nick: Comparison of I-SceI Single- and Double-Strand Break-Induced Recombination in Yeast and Human Cells
February 18, 2014 Samantha Stuckey Katz, Frederick Gimble, Francesca Storici
Image credit
10.1371/journal.pone.0085383

PLOS ONE Epigenetic Alterations at Genomic Loci Modified by Gene Targeting in Arabidopsis thaliana
December 26, 2013 Michal Lieberman-Lazarovich, Cathy Melamed-Bessudo, Sylvia de Pater, Avraham Levy
Image credit
10.1371/journal.pone.0087703

PLOS ONE Targeted Methylation of the Epithelial Cell Adhesion Molecule (EpCAM) Promoter to Silence Its Expression in Ovarian Cancer Cells
January 29, 2014 Suneetha Nunna, Richard Reinhardt, Sergey Ragozin, Albert Jeltsch
Image credit
10.1371/journal.pone.0074254

PLOS ONE The Design and In Vivo Evaluation of Engineered I-OnuI-Based Enzymes for HEG Gene Drive
September 10, 2013 Yuk-Sang Chan, Ryo Takeuchi, Jordan Jarjour, David Huen, Barry Stoddard, Steven Russell
Image credit
10.1371/journal.pone.0062054

PLOS ONE A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs
April 29, 2013 Karamjit Dolt, Melanie Lawrence, Eve Miller-Hodges, Joan Slight, Anna Thornburn, Paul Devenney, Peter Hohenstein
Image credit
10.1371/journal.pone.0053217

PLOS ONE High Frequency Targeted Mutagenesis Using Engineered Endonucleases and DNA-End Processing Enzymes
January 24, 2013 Fabien Delacôte, Christophe Perez, Valérie Guyot, Marianne Duhamel, Christelle Rochon, Nathalie Ollivier, Rachel Macmaster, George Silva, Frédéric Paques, Fayza Daboussi, Phillipe Duchateau
Image credit
10.1371/journal.pone.0059400

PLOS ONE p53 Gene Targeting by Homologous Recombination in Fish ES Cells
March 19, 2013 Yan Yan, Ni Hong, Tiansheng Chen, Mingyou Li, Tiansu Wang, Guijun Guan, Yongkang Qiao, Songlin Chen, Manfred Schartl, Chang-Ming Li, Yunhan Hong
Image credit
10.1371/journal.pone.0053492

PLOS ONE Engineering HIV-1-Resistant T-Cells from Short-Hairpin RNA-Expressing Hematopoietic Stem/Progenitor Cells in Humanized BLT Mice
December 31, 2012 Gene-Errol Ringpis, Saki Shimizu, Hubert Arokium, Joanna Camba-Colón, Maria Carroll, Ruth Cortado, Yiming Xie, Patrick Kim, Anna Sahakyan, Emily Lowe, Munetoshi Narukawa, Fadi Kandarian, Bryan Burke, Geoff Symonds, Dong Sung An, Irvin Chen, Masakazu Kamata
Image credit
10.1371/journal.pone.0054130

PLOS ONE Optimising Homing Endonuclease Gene Drive Performance in a Semi-Refractory Species: The Drosophila melanogaster Experience
January 18, 2013 Yuk-Sang Chan, David Huen, Ruth Glauert, Eleanor Whiteway, Steven Russell
Image credit
10.1371/journal.pone.0047389

PLOS ONE Simple Monitoring of Gene Targeting Efficiency in Human Somatic Cell Lines Using the PIGA Gene
October 8, 2012 Sivasundaram Karnan, Yuko Konishi, Akinobu Ota, Miyuki Takahashi, Lkhagvasuren Damdindorj, Yoshitaka Hosokawa, Hiroyuki Konishi
Image credit
10.1371/journal.pone.0042905

PLOS ONE Transformation with Oligonucleotides Creating Clustered Changes in the Yeast Genome
August 14, 2012 Gina Rodriguez, Joseph Song, Gray Crouse
Image credit
10.1371/journal.pone.0039895

PLOS ONE I-SceI-Mediated Double-Strand Break Does Not Increase the Frequency of Homologous Recombination at the Dct Locus in Mouse Embryonic Stem Cells
June 26, 2012 Myriam Fenina, Dominique Simon-Chazottes, Sandrine Vandormael-Pournin, Jihane Soueid, Francina Langa, Michel Cohen-Tannoudji, Bruno Bernard, Jean-Jacques Panthier
Image credit
10.1371/journal.pone.0037071

PLOS ONE Targeting of the Human Coagulation Factor IX Gene at rDNA Locus of Human Embryonic Stem Cells
May 16, 2012 Xionghao Liu, Yong Wu, Zhuo Li, Junlin Yang, Jinfeng Xue, Youjin Hu, Mai Feng, Wenbin Niu, Qiurui Yang, Ming Lei, Jiahui Xia, Lingqian Wu, Desheng Liang
Image credit
10.1371/journal.pone.0031997

PLOS ONE W::Neo: A Novel Dual-Selection Marker for High Efficiency Gene Targeting in Drosophila
February 13, 2012 Wenke Zhou, Juan Huang, Annie Watson, Yang Hong
Image credit
10.1371/journal.pone.0032646

PLOS ONE A Rapid FACS-Based Strategy to Isolate Human Gene Knockin and Knockout Clones
February 29, 2012 João Mata, Telma Lopes, Rui Gardner, Lars Jansen
Image credit
10.1371/journal.pone.0036697

PLOS ONE Stable Gene Targeting in Human Cells Using Single-Strand Oligonucleotides with Modified Bases
May 14, 2012 Xavier Rios, Adrian Briggs, Danos Chistodoulou, Josh Gorham, Jonathan Seidman, George Church
Image credit
10.1371/journal.pone.0028283

PLOS ONE Inducible Gene Manipulations in Brain Serotonergic Neurons of Transgenic Rats
November 29, 2011 Tillmann Weber, Kai Schönig, Björn Tews, Dusan Bartsch
Image credit
10.1371/journal.pone.0026422

PLOS ONE A New Powerful Method for Site-Specific Transgene Stabilization Based on Chromosomal Double-Strand Break Repair
October 17, 2011 Artem Tkachuk, Maria Kim, Oksana Kravchuk, Mikhail Savitsky
Image credit
10.1371/journal.pone.0023981

PLOS ONE DNA Nicks Promote Efficient and Safe Targeted Gene Correction
September 1, 2011 Luther Davis, Nancy Maizels
Image credit
10.1371/journal.pone.0019722

PLOS ONE Assembly of Designer TAL Effectors by Golden Gate Cloning
May 19, 2011 Ernst Weber, Ramona Gruetzner, Stefan Werner, Carola Engler, Sylvestre Marillonnet
Image credit
10.1371/journal.pone.0019794

PLOS ONE Non-Integrative Lentivirus Drives High-Frequency cre-Mediated Cassette Exchange in Human Cells
May 23, 2011 Raul Torres, Aida Garcia, Monica Paya, JUAN Ramirez
Image credit
10.1371/journal.pone.0019509

PLOS ONE Transcriptional Activators of Human Genes with Programmable DNA-Specificity
May 19, 2011 Rene Geißler, Heidi Scholze, Simone Hahn, Jana Streubel, Ulla Bonas, Sven-Erik Behrens, Jens Boch
Image credit
10.1371/journal.pone.0019537

PLOS ONE Zinc Finger Recombinases with Adaptable DNA Sequence Specificity
April 29, 2011 Chris Proudfoot, Arlene McPherson, Andreas Kolb, Marshall Stark
Image credit
10.1371/journal.pone.0016825

PLOS ONE Successful Targeting and Disruption of an Integrated Reporter Lentivirus Using the Engineered Homing Endonuclease Y2 I-AniI
February 9, 2011 Martine Aubert, Byoung Ryu, Lindsey Banks, David Rawlings, Andrew Scharenberg, Keith R Jerome
Image credit
10.1371/journal.pone.0004640

PLOS ONE Temporally-Controlled Site-Specific Recombination in Zebrafish
February 27, 2009 Stefan Hans, Jan Kaslin, Dorian Freudenreich, Michael Brand
Image credit
10.1371/journal.pone.0003121

PLOS ONE Group II Intron-Based Gene Targeting Reactions in Eukaryotes
September 1, 2008 Marta Mastroianni, Kazuo Watanabe, Travis B. White, Fanglei Zhuang, Jamie Vernon, Manabu Matsuura, John Wallingford, Alan M. Lambowitz

Synthetic Biology Collection

To browse other Synthetic Biology research collections

Browse

PLOS SynBio Community

Visit the community page to read about synbio trends, events & recent research

Learn More

PLOS Synbio